Syringe Filter Impact Study
10/7/24
Attached is the filter impact study that we recently performed. Here are some key takeaways :
1. Filter Size Matters: The study found that using a 4mm syringe filter results in significantly less volumetric loss compared to a 13mm filter. Specifically, the 4mm filter loses about 5-7 units, while the 13mm filter loses approximately 29 units when filtering a reconstituted peptide solution.
2. Air Purge Recovery: For the 4mm filter, performing air purges can help recover some of the lost volume, on average, around 2.2 units of the 7.3 units lost can be recovered through this method, reducing the overall loss. Air purging was not effective on 13mm filter.
3. Minimal Impact on Concentration: The impact of filtration on peptide concentration is minimal. For both 4mm and 13mm filters, the changes in concentration were not significant enough to affect practical use, suggesting that filtration primarily affects volume rather than peptide concentration. Interestingly, the study observed a slight increase in peptide concentration after filtration in some cases. This may suggest that the filter retains some water while allowing the peptide to pass through, potentially increasing peptide concentration in the final solution.
